With regard to the beads, the singlet flow count beads in the stemkit are detected in a bivariate plot of fl3 ecd versus time on beckman coulter xl and fc500 instruments 12,18,19,21,22. The cell or particle scatters the light into forward and side scatter signals indicators of the particles size, shape and structure. Flow cytometry analysis using the becton dickinson facs. None of the equipment posted for sale on is owned by medwow, should you have any questions. Comparison of two singleplatform ishagebased cd34 enumeration protocols on bd facscalibur and facscanto flow cytometers article pdf available in cytotherapy 115. This modular system features advanced capabilities, such as the sorting and fl4 options in an easytouse system. Bd biosciences 2 laser facscalibur flow cytometer marshall. Bd facscalibur the flow cytometer for your routine cell analysis needs the bd facscalibur flow cytometry system is another bd first. Operate them only as directed in the reference manual.
Page 1 bd facscalibur instructions for use for in vitro diagnostic use part no. The instrument is designed to support a wide range of. Bectondickinson facscalibur flow cytometer instructions. The bd facscalibur system is an automated benchtop flow cytometry system that offers three or fourcolor capability and can perform both cellular analysis and optional cell sorting. The hts option supports 96 u, v, and flatbottom plates as well as. The fundamental concept is that cells flow one at a time through a region of interrogation where multiple biophysical properties of each cell can be measured at rates of over cells per second. Becton dickinson facscalibur cytometer manufacturer. The bd biosciences facscalibur is a 2 laser flow cytometer that can be used to simultaneously analyze up to 4 fluorescent parameters. The system supports a wide variety of research and clinical. Special procedures and troubleshooting beckman coulter. The bd facscalibur platform offers a unique modular approach to flow cytometry and allows users to perform both cell analysis and cell sorting in an innovative. Laser light is focused onto the flow cell by a focusing. Used bd biosciences bd facscalibur flow cytometer flow. Bd accuri c6 plus flow cytometer with its compact 11 x 14.
Equipment for sale category or equipment item facscaliburflowcytometer3colors not found. Because the placement of the sample core stream within the cuvette flow cell and the laser alignment are fixed, daily instrument optimization of the. It designed for applications that range from routine clinical to advanced research, analyzing single cells as they pass through a focused laser beam. The flow cytometer is used to measure physical and chemical properties of cells or particles in suspension as they flow, in single file, through a beam of light. Bd facscalibur flow cytometry system with hts autosampler. Do not perform instrument maintenance or service except as specifically stated. Bd facscalibur instructions for use boston university. The fl4 option provides a second 635nm, reddiode laser and an additional detector.
These instruments include a bectondickinson bd facsvantagese high speed cell sorter, and a bd facscalibur bench top flow cytometer. Designed specifically to support a wide range of applications, the. The gold standard that continues to evolve the bd facscalibur flow cytometer is another bd biosciences first. As a modular option for new or existing bd facscalibur flow cytometers, the hts option is designed to speed through a variety of microtiter plates in less than 15 minutes and support a wide range of applications including the bd cytometric bead array assay. This unit details the operation of a facs calibur flow cytometer for cell analysis. Facscalibur system users guide v preface facscalibur is the becton dickinson immunocytometry systems bdis modular benchtop flow cytometer designed for applications ranging from routine clinical to advanced research. Vortex the culture tube, make a dilution of samples in 1ml tbs1x try 20l in 1ml, vortex and put the tube on the facscalibur. Stratedigm offers seamless integration of automation, highthroughput technology, instrumentation, and software that delivers the utmost in productivity. The instrument is designed to support a wide range of applications with high resolution and sensitivity of both fluorescence and scatter. The facscalibur system is a modular, benchtop flow cytometer from becton dickinson immunocytometry system bdis that is easily modified based on research needs. The bd facscalibur flow cytometer identifies and enumerates lymphocyte. Documents document view page bd facscalibur cytometer. Bd facscalibur flow cytometers, the hts option is designed to speed through a variety of microtiter plates in less than 15 minutes and support a wide range of applications including the bd cytometric bead array assay. Flowform007 bd facscalibur flow cytometer instrument qc log stem cell laboratory, dumc durham, nc confidential printed by.
Blizard institute flow cytometry core facility facs canto ii quick user guide page 5 1. Becton dickinson facscalibur flow cytometer with spa ii system compared to an aquios cl flow cytometer the flow cytometry laboratory at university of texas is situated on an 84acre campus with four schools, three. It has a 488nm blue laser and 637nm red laser, with 4 filters 525bp, 585 bp, 670 lp, 650 lp. The bd facscalibur system is an automated benchtop flow cytometry system that offers three or fourcolor capability and can perform both cellular analysis. If you are not familiar with the windows operating system, refer to the documentation provided with your computer. Laboratory equipment for sale scientific equipment repair. The becton dickinson biosciences facscalibur flow cytometer offers a unique approach to flow cytometry, allowing users to perform both cell analysis and cell sorting in a single system, providing flexibility for a variety of research and clinical applications. Bd facsaria flow cytometer by janine sharpe next generation flow cytometry highspeed sorter the bd facsaria cell sorter sets a new standard for highperformance flow. The becton dickinson facscalibur flow cytometer offers a unique approach to flow cytometry, allowing users to perform both cell. Bd facscalibur flow cytometer from bd biosciences selectscience.
Bd facscalibur flow cytometer maintenance log all dates. The system supports a wide variety of research and clinical applications and is complemented by a broad suite of intuitive software solutions to streamline analysis for a wide range of applications including enumeration of. Combined with your choice of sample loader, a wide variety of reagents, and powerful software, the bd facscalibur. The bd facscalibur flow cytometer is another bd biosciences first. Facscalibur is the becton dickinson immunocytometry systems bdis modular benchtop flow cytometer designed for applications ranging from routine clinical. The bd facscanto flow cytometer and the bd facs loader are equipped with safety features for your protection. University of texas medical branch utmb, galveston, texas, usa flow cytometry equipment. The term flow cytometry derives from the measurement meter of single cells cyto as they flow past a series of detectors. This information can be used to individually sort or separate subpopulations of cells. The operation of the becton dickinson facs calibur and cellquest software version 3.
Two major advantages of the cuvette flow cell design are improved light excitation and collection optics. Purchase a stratedigm benchtop flow cytometer and be confident that you have made the best possible flow cytometry investment. If you are not familiar with the windows operating. Becton dickinson bd facscalibur flow cytometer tags. Check that the cytometer is clean no event in 30secondes, doing a start but let the set up ticked and then pauseabort.
From the drop down menu under cytometer, select the. Bd facs calibur flow cytometer university of new brunswick. Jan 20, 2016 bd facscalibur flow cytometer available at the lab world group. Pdf comparison of two singleplatform ishagebased cd34. Bd facscalibur instructions for use bd biosciences. The becton dickinson facscalibur flow cytometry system is high performance, with superior sensitivity, maximum flexibility and minimal compensation. Based on an entirely new design in instrumentation, the bd facsaria instrument is the first benchtop sorter that incorporates a fixedalignment cuvette flow cell. Flow cytometry flow cytometry is the technical process that allows for the individual measurements of cell fluorescence and light scattering. It is a tricolor, automated system that is capable of analyzing a wide variety of. This is every users responsibility so please pay attention to these guidelines for the sake of yourself and all other facscalibur users. Bd facscalibur flow cytometry system bd biosciences.
Becton dickinson facscalibur flow cytometer with spa ii system compared to an aquios cl. Small benchtop flow cytometers from stratedigm, inc. Becton dickinson facscalibur flow cytometry system with hts autosampler available at the lab world group. A november 2007 bd biosciences benex limited canada japan nippon becton dickinson san jose, ca 9511807 bay k 1 ad toll free 888 2590187 company, ltd. The becton dickinson facscalibur flow cytometer is an innovative single benchtop system, combining cell analysis and employing a customizable approach to flow cytometry analysis. Bd facscalibur flow cytometer new costs over 100k predecessor to. In our lab, this instrument is primarily used for diagnostic samples. If you operate this instrument in any way not specified in the. The becton dickinson biosciences facscalibur flow cytometer offers a unique approach to flow cytometry, allowing. It analyzes cells as they pass through a focused laser beam one at a time in a moving. Jul 31, 2007 in flow cytometry, only 4 hours are necessary to prepare the samples and roughly 1 hour to acquire and analyse the data. Flow cytometer facscalibur and facsvantage flow sorter. Bd biosciences 2 laser facscalibur flow cytometer w hts autosampler. The facsvantagese will sort or isolate subpopulations of cells from a.
With regard to the beads, the singlet flowcount beads in the stemkit are detected in a bivariate plot of fl3 ecd versus time on beckman coulter xl and fc500 instruments. Coulter epics xl flow cytometer coulter epics xlmcl flow cytometer system ii software special procedures and troubleshooting tm cytometer laser sample flow low ready on med high fs ss aux fl4 fl3 fl1 fl2 pn 4237296ca september 2010 beckman coulter, inc. A bd facscan system the facscan system is an automated. Flow cytometry is not the most sensitive technique but the sensitivity is largely acceptable 1. Start, control if the number of eventsseconds is pdf available in cytotherapy 115.
Flow cytometry analysis using the becton dickinson facs calibur. A flow cytometry technique to study intracellular signals nf. Bd facscalibur flow cytometer available at the lab world group. The bd facscanto ii flow cytometer reference manual assumes you have a working knowledge of basic microsoft windows operation. The becton dickinson facscalibur flow cytometer offers a unique approach to flow cytometry, allowing users to perform both cell analysis and cell sorting in a single system, providing flexibility for a variety of research and clinical applications. Bd facsaria flow cytometer by janine sharpe next generation flow cytometry highspeed sorter the bd facsaria cell sorter sets a new standard for highperformance flow cytometry. Jan 25, 2016 the bd facscalibur allows users to perform cell analysis for a wide range of applications including enumeration of lymphocyte subsets, stem cells, residual white blood cells, and reticulocytes. Notes on facscalibur use and troubleshooting procedures. Facscalibur training flow cytometry cytometrythe characterization and measurement of cells and cellular constituents flow to issue or move in a stream flow cytometry, then, can be defined as the characterization and measurement of cells and cellular constituents as they travel in a fluid stream. May, 2016 becton dickinson facscalibur flow cytometry system with hts autosampler available at the lab world group. Purpose of this guide who should read this guide this.
Bd biosciences 2 laser facscalibur flow cytometer manual warranty information laboratory controls offers a best in the business full 90 day warranty on all refurbished items. Documents document view page bd facscalibur cytometer manual. Flow cytometer instruments by apogee flow systems manufacturers of automated science laboratory equipment for research and commercial use speeds up cell counting, particle identification, bacteria classification or leukocyte classification in military, medical, and microbiology labs. It is the most feasible technique, however, the use of the. With its high speed analysis and multiparameter triggering system, the bd facscalibur flow cytometer is capable of rareevent detection like the characterization of subpopulation of stem cells. As a modular option for new or existing bd facscalibur flow cytometers, the hts option is designed to speed through. Not for use in diagnostic or therapeutic procedures. Flow cytometry is a process in which such measurements are made while. In flow cytometry, only 4 hours are necessary to prepare the samples and roughly 1 hour to acquire and analyse the data. A cellquest users guide pdf is on the computer desktop feel free to copy it onto. Flow cytometer filter guide dyes filters mirrors 2 laser blueviolet 10 color 3 laser bluevioletred 12 color 3 laser blueviolet yellowgreen 12 color 3 laser blueviolet ultraviolet 12 color blue laser 488 nm bd horizon brilliant blue 515, fitc 53030 505 lp pe 57525 550 lp bd horizon pecf594 61020 600 lp.
Schedule time on a cytometer facs or calibur by filling in the calendar at the flow lab. The becton dickinson facscalibur flow cytometer is an innovative single benchtop system, combining cell analysis and employing a customizable approach to flow cytometry analysis through modular components. Instrument received a full pm and is ready for resale. The bd facscalibur platform allows users to perform both cell analysis and cell sorting in a single benchtop system.
All instruments are reconditioned and include a 90 day warranty on parts and labor. It analyzes cells as they pass through a focused laser beam one at. Cell flow instruments and the technical support and expertise will be available to the lbnl and external research community. Bd facs canto ii flow cytometer quick user guide v. Flow cytometer filter guide dyes filters mirrors 2 laser blueviolet 10 color 3 laser bluevioletred 12 color 3 laser blueviolet yellowgreen 12 color 3 laser blueviolet. Becton dickinson facscalibur flow cytometry system youtube. Guide to using bd cba kits with the bd facscalibur system. The intuitive bd facscalibur flow cytometer instrument can be upgraded to keep up with the laboratorys sorting and automation needs. Bd facscalibur bd pdf catalogs technical documentation. The bd facscalibur allows users to perform cell analysis for a wide range of applications including enumeration of lymphocyte subsets, stem cells, residual white blood. Flow cytometer instruments by apogee flow systems manufacturers of automated science laboratory equipment for research and commercial use speeds up cell counting, particle. Facscalibur training flow cytometry cytometrythe characterization and measurement of cells and cellular constituents flowto issue or move in a stream flow cytometry, then, can be. This process is performed at rates of thousands of cells per second.
Responsibilities of all flow cytometer users these guidelines suggest ways in which all users can help to ensure that the facscalibur machines remain operational and in excellent condition. Because the placement of the sample core stream within the cuvette flow cell and the laser alignment are fixed, daily instrument optimization of the cytometer is no longer necessary. Bd facscalibur 2 laser, 4 color flow cytometer marshall. Dont grossly overbook since we have to pay for both reserved time and the time actually used which ever is greater. Manufacturer specifications facscalibur, becton dickinson please note. Purpose of this guide who should read this guide this guide provides setup and acquisition instructions for using a bd facscalibur flow cytometer single or dual laser to acquire data obtained with a. On bd facscanto and bd facscalibur instruments, they are detected on a bivariate plot of forward scatter versus time,18,19,21,22.
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